Laboratory of biomedical microbiology and immunology

Department of microbiology and immunology

 

The history

    The Laboratory of Biomedical Microbiology and Immunology (LBMI) was founded in 1996, the 15th of March. Two organizations, University of veterinary medicine and pharmacy in Košice and Institute of Neuroimmunology, Slovak academy of science, came together with a common goal – “establishment of a sophisticated research unit in the field on immunogenomics and immunoproteomics”.


The present

    Since its foundation the LBMI is assuring the excellence in research and education. The research activities of the LBMI concern the exploration of host-pathogen interactions.  This agenda covers a broad range of highly specialized and specific research activities like: a) effect of mutations in the genes of innate immunity on the susceptibility of the host to pathogens b) exploration of host specificity of pathogen and c) exploration of immune evasive mechanisms of pathogens.

    To achieve the goals state of the art techniques used in the LBMI are: different nucleic acid isolation techniques including isolation of genomic DNA, RNA as well as plasmid; nucleic acid amplification (NAAs) like end point PCR, real time PCR, multiplex assays, mutation detection techniques like SSCP, DGGE, high resolution melting (HRM) assays; direct DNA sequencing, gene expression assays like qRT-PCRs, transfection techniques, in-vitro recombinant protein design and production, protein-protein interaction assays like M2H, affinity ligand binding, pull-down assays and cell culturing techniques.


The main research topic of LBMI:

  1. interactions between Borrelia and blood-brain barrier

  2. proteomics aspects of Francisella:blood-brain barrier interactions

  3. human complement system interacting with Borrelia and Francisella

  4. trypanosomial interaction with human complement system

  5. the relationship between complement system and Trypanosoma

  6. revealing of new proteins involving in Trypanosoma:BMEC interactions

  7. development of proteins array for vector-borne diseases

  8. development of nanoantibodies against Borrelia