Laboratory of biomedical microbiology and immunology

DNA Sequencing and genotyping

 


Our team provides sequencing and genotyping analysis of your samples. We need only your template (purified PCR product, if you cannot purified the product, we can manage this purification). We will sequence your samples on ABI PRISM 3100-Avant Genetic Analyzer.



How we need sample:


          Minimal volume of sample: 10 ul


          Quality of sample: we recommend to purify the PCR product from agarose gel or purification directly from PCR product, if there is any non-specific products in your PCR product. We are recommend to use Qiagen, Promega, Jena or similar.


          Volume of primer: 10 ul of each primer with the concentration 10 pmol.


          Maximal size of product: we can provide sequencing of max. 500 bp of each forward and reverse primer.


• Download this form (click here), fill it carefully and bring it to us along with samples or send by mail - martina.cepkova@uvlf.sk

     


Service details


Delivery: 2-7 days


Output format: sequence and chromatogram


Price: 9 Euro per sample (price is including sequencing with 1 primer and tax). In case of bulk samples (50 samples or more) a discount will be given. For discount Please contact - +421915984604,  mangesh.bhide@uvlf.sk or bhidemangesh@gmail.com


After sequencing, we are able to analyze your sequence and determine the gene, site of mutation, identify the pathogen and isolate from the sample. The price for genotyping is on request.



Invoice and quoataion query

For invoice and other query contact ing. Viera Kopcakova or ing. Martina Cepkova (+421918180633), viera.kopcakova@uvlf.sk or  martina.cepkova@uvlf.sk